Journal: Cells

Article Title: Sprouty2 Regulates Endocytosis and Degradation of Fibroblast Growth Factor Receptor 1 in Glioblastoma Cells

doi: 10.3390/cells13231967

Figure Lengend Snippet: Primary antibodies used for immunostaining and Western blot experiments.

Article Snippet: Clathrin heavy chain (D3C6) XP , Cell signaling , 4796.

Techniques: Immunostaining, Western Blot, Ubiquitin Proteomics

Journal: Cells

Article Title: Sprouty2 Regulates Endocytosis and Degradation of Fibroblast Growth Factor Receptor 1 in Glioblastoma Cells

doi: 10.3390/cells13231967

Figure Lengend Snippet: SPRY2 inhibits colocalization of clathrin with FGFR1 and FGF2 in U251 and SF126 cells. ( A ) U251 control cells with low endogenous SPRY2 level and U251 cells with SPRY2-OE were transfected with FGFR1-EGFP, treated with FGF2-Cy3 for 30 min, and immunostained against clathrin. Whole-cell analysis of confocal images revealed no change in the number of clathrin vesicles per cell (blue) after SPRY2-OE. The colocalization of clathrin (blue) with FGFR1 (green; colocalization with clathrin = turquoise) and FGF2 (red; colocalization with clathrin = magenta) was reduced in response to SPRY2-OE. N = 6 experiments, mean ± SEM. * p < 0.05. ( B ) SF126 control cells with high endogenous SPRY2 content and SF126 cells with shSPRY2 were transfected with FGFR1-EGFP, treated with FGF2-Cy3 for 30 min, and immunostained against clathrin. The number of clathrin vesicles per cell (blue) was not altered with shSPRY2. The colocalization of clathrin (blue) with FGFR1 (green; colocalization with clathrin = turquoise) and FGF2 (red; colocalization with clathrin = magenta) was enhanced with shSPRY2. N = 5 experiments, mean ± SEM. ** p < 0.01. White, bold arrowheads indicate cell surface localization of FGFR1 and FGF2. Yellow arrowheads mark internalized FGFR1 and FGF2 vesicles colocalizing with clathrin. Scale bar = 4 µm.

Article Snippet: Clathrin heavy chain (D3C6) XP , Cell signaling , 4796.

Techniques: Control, Transfection, Cell Analysis